Wednesday, May 30, 2012

PO-248: TSE infectivity survives burial for five years with little reduction in titer

PO-248: TSE infectivity survives burial for five years with little reduction in titer


Allister Smith, Robert Somerville, Karen Fernie The Roslin Institute and R(D)SVS; University of Edinburgh; Edinburgh, UK


BSE infected animals, BSE-contaminated materials and other sources of TSE (prion) infection, such as carcasses from scrapie infected sheep, CWD infected deer and cadavers of individuals infected with CJD may all end up in the environment through burial or other methods of disposal. They may continue to act as a reservoir of TSE infectivity if cattle or other susceptible animals were to be exposed to these sources in the future. In order to address these concerns, we performed two large scale demonstration experiments under field conditions which were designed to mimic some of the ways by which TSE infected materials may have been disposed of. The project examined the fate of TSE infectivity over a period of five years in two scenarios; when the infectivity was contained within bovine heads and when the infectivity was buried without any containment. Two soil types were compared: a sandy loam and a clay loam. We used the 301V TSE strain which was derived by serial passage of BSE in VM mice.


TSE infectivity was recovered from all the heads exhumed annually for five years from both types of soil, with little reduction in the amount of infectivity throughout the period of the experiment. Small amounts of infectivity were found in the soil immediately surrounding the heads, but not in samples remote from them. Similarly there was no evidence of significant lateral movement of infectivity from the buried bolus. However large amounts of TSE infectivity were recovered at the site of burial of both boluses. There was limited vertical upward movement of infectivity from the bolus buried in clay soil and downward movement from the bolus buried in sandy soil.


Now that these experiments are completed we conclude that TSE infectivity is likely to survive burial for long periods of time with minimal loss of infectivity and restricted movement from the site of burial. These experiments emphasize that the environment is a viable reservoir for retaining large quantities of TSE infectivity, and reinforce the importance of risk assessment when disposing of this type of infectious material.






meanwhile, back at the ranch with larry, curly, and mo at USDA ET AL ON BSE ALABAMA STYLE



Epidemiology Update March 23, 2006



As of today, 13 locations and 32 movements of cattle have been examined with 27 of those being substantially completed. Additional investigations of locations and herds will continue. In addition, state and federal officials have confirmed that a black bull calf was born in 2005 to the index animal (the red cow). The calf was taken by the owner to a local stockyard in July 2005 where the calf died. The calf was appropriately disposed of in a local landfill and did not enter the human or animal food chain. http://www.aphis.usda.gov/newsroom/hot_issues/bse/bse_al_epi-update.shtml



> The calf was appropriately disposed of in a local



> landfill and did not enter the human or animal food chain.



well, back at the ranch with larry, curly and mo heading up the USDA et al, what would you expect, nothing less than shoot, shovel and shut the hell up. no mad cow in USA, feed ban working, no civil war in Iraq either.



SNIP...SEE FULL TEXT ;



meanwhile, back at the ranch with larry, curly, and mo at USDA ET AL ON BSE ALABAMA STYLE









Thursday, February 17, 2011





Environmental Sources of Scrapie Prions




http://scrapie-usa.blogspot.com/2011/02/environmental-sources-of-scrapie-prions.html




Friday, February 25, 2011


Soil clay content underlies prion infection odds Soil clay content underlies prion infection odds


http://chronic-wasting-disease.blogspot.com/2011/02/soil-clay-content-underlies-prion.html




Saturday, March 10, 2012


CWD, GAME FARMS, urine, feces, soil, lichens, and banned mad cow protein feed CUSTOM MADE for deer and elk


http://chronic-wasting-disease.blogspot.com/2012/03/cwd-game-farms-urine-feces-soil-lichens.html



Friday, May 18, 2012






Update from APHIS Regarding a Detection of Bovine Spongiform Encephalopathy (BSE) in the United States Friday May 18, 2012









Friday, May 25, 2012





R-CALF USDA’s New BSE Rule Eliminates Important Protections Needed to Prevent BSE Spread









Saturday, May 26, 2012




Are USDA assurances on mad cow case 'gross oversimplification'?











TSS


No comments: